1x first strand buffer  (Thermo Fisher)

Journal: STAR Protocols

Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

doi: 10.1016/j.xpro.2025.104275

Figure Lengend Snippet: RT mix-II

Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

Techniques:

Journal: Nature Communications

Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development

doi: 10.1038/s41467-026-69176-8

Figure Lengend Snippet: a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

Article Snippet: Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers.

Techniques: Control, Synthesized, Amplification, Derivative Assay, Purification, Sequencing, Modification, Biomarker Discovery